trizol reagent (tiangen biotech co)

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tiangen biotech co trizol reagent
Trizol Reagent, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 98/100, based on 1449 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dp424/TRNzol+Universal+Reagent/pmc13042271-77-11-17
Average 98 stars, based on 1449 article reviews

trizol reagent - by Bioz Stars, 2026-10

98/100 stars

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Extraction:

Article Title: A universal fluorescent lateral flow strip approach based on molecular beacon-assisted rolling circle amplification for point-of-care nucleic acid detection
Article Snippet: .. The total extraction kits DP304 and DP424, purchased from Tiangen Biotech (Beijing, China), were used to extract total DNA and RNA from clinical throat swab samples following the manufacturer’s instructions. ..

Purification:

Article Title: Impact of Faba Bean (Vicia faba L.) Diet and Subsequent Withdrawal on GIFT Tilapia (Oreochromis niloticus) Muscle Quality
Article Snippet: Alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), total cholesterol (TC), and high-density lipoprotein cholesterol (HDLC) were detected by an automatic biochemical analyzer HITACHI 7160 (Hitachi, Ltd.) according to a previous study [31]. .. Total RNA was extracted and purified using a commercial kit (No. DP424; Tiangen Biotech Co., Ltd., Beijing, China) and verified by 1.5% agarose gel electrophoresis, following methods described in previous studies [30,37]. cDNA was synthesized from 1 μg of total RNA using reverse transcriptase and a commercial kit (No. RR047B; TaKaRa, Osaka, Japan), according to the manufacturer’s instructions. ..

Agarose Gel Electrophoresis:

Synthesized:

Reverse Transcription:

Article Title: Regulated transformation system (RTS): sddi-mediated programmable shut-off and mode switching of base editors.
Article Snippet: ACBE-RTS expression was confirmed by western blot (antiCas9, Abcam, UK, ab204448; anti-Tubulin, Beyotime, China, AF2827). .. Quantitative PCR Total RNA was extracted (TRNzol Universal, Tiangen, China, DP424), reverse-transcribed (FastKing RT Kit, Tiangen, China, KR116), and quantified (NanoDropTM 1000, Thermo Fisher Scientific, USA). .. PRRSV-N mRNA levels were assayed via quantitative PCR (qPCR) (Talent PreMix, Tiangen, China, FP209; q225, Novogene, China).

Article Title: Regulated transformation system (RTS): sddi-mediated programmable shut-off and mode switching of base editors
Article Snippet: ACBE-RTS expression was confirmed by western blot (anti-Cas9, Abcam, UK, ab204448; anti-Tubulin, Beyotime, China, AF2827). .. Total RNA was extracted (TRNzol Universal, Tiangen, China, DP424), reverse-transcribed (FastKing RT Kit, Tiangen, China, KR116), and quantified (NanoDropTM 1000, Thermo Fisher Scientific, USA). .. PRRSV-N mRNA levels were assayed via quantitative PCR (qPCR) (Talent PreMix, Tiangen, China, FP209; q225, Novogene, China).

Real-time Polymerase Chain Reaction:

Isolation:

Article Title: Dissolvable microneedle-assisted transdermal administration of diacerein nanoparticles achieved satisfactory therapeutic effects in tendon-bone insertion repair by reducing oxidative stress and inflammation
Article Snippet: Total RNA preparation and subsequent RNA-seq library construction are executed with the APExBIO Technology LLC (Shanghai, China) service. .. Briefly, total RNA is isolated using a commercial kit (Tiangen Biotech, DP424), and RNA libraries are established via an RNA cleaning and concentration kit (APExBIO Technology LLC, K1159) after quality inspection and purity testing. .. The integrity of the RNA samples is confirmed through the utilization of an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA).

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